Analysis Note

ControlHeLa cell lysate

Application

Research Sub CategoryTranscription Factors

Research CategoryEpigenetics & Nuclear Function

Immunohistochemistry Analysis: A representative lot was used by an independent laboratory in IH. (Kinross, K., et al. (2006). Blood. 108(3):886-895.)

Anti-E2F4 Antibody, clone LLF4-2 is a Mouse Monoclonal Antibody for detection of E2F4 also known as Transcription factor E2F4, E2F-4 & has been validated in WB & IHC.

Disclaimer

Unless otherwise stated in our catalog or other company documentation accompanying the product(s), our products are intended for research use only and are not to be used for any other purpose, which includes but is not limited to, unauthorized commercial uses, in vitro diagnostic uses, ex vivo or in vivo therapeutic uses or any type of consumption or application to humans or animals.

General description

E2F4 is the most common E2F family member in a number of cell types. E2F4 deficiency has been shown to cause a decrease in the thickness of the gut epithelium, decrease in maturation of hematopoietic cell types and an increased rate of apoptosis, suggesting that E2F4 plays a role in normal maturation of several cell types. E2F4 has also been shown to be essential for the normal development of airway epithelium and early eye development. Other data has implicated E2F4 during normal heart development, specifically cardiomyocyte proliferation.

Immunogen

Histidine-tagged recombinant protein corresponding to human E2F4.

Other Notes

Concentration: Please refer to the Certificate of Analysis for the lot-specific concentration.

Physical form

Purified mouse monoclonal IgG1κ in buffer containing 0.1 M Tris-Glycine (pH 7.4), 150 mM NaCl with 0.05% sodium azide.

Protein G

Format: Purified

Quality

Evaluated by Western Blot in HeLa cell lysate.

Western Blot Analysis: 0.5 µg/mL of this antibody detected E2F4 on 10 µg of HeLa cell lysate.

Storage and Stability

Stable for 1 year at 2-8°C from date of receipt.

Target description

Bands may be detected at ~45 and ~60 kDA